Human Anti-LYZ Recombinant Antibody (clone HuLys11)
CAT#: PABW-023
Recombinant Humanized Antibody (HuLys11) is capable of binding to Chicken LYZ, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Chicken LYZ mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-Chicken LYZ mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Tested Data
Gene Expression
ELISA
Figure 1 Anti-LYZ Recombinant Antibody (PABW-023) in ELISA
ELISA analysis of PABW-023 was performed by coating with chicken egg white lysozyme (0.5 μg/well). Then blocked with BSA and incubated with PABW-023. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-LYZ Recombinant Antibody (PABW-023) in WB
Western blot analysis of PABW-023 was performed with chicken egg white lysozyme in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with PABW-023 (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
DB
Figure 3 Anti-LYZ Recombinant Antibody (PABW-023) in DB
Dot Blot analysis of PABW-023 was performed by coating with chicken egg white lysozyme.
PABW-023 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
NC: Negative control PC: Positive control
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm, the Golgi apparatus & actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/66182/1763_E3_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human salivary gland
High expression. RNA expression: 21115.5 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_1_val_selected.jpg
Normal Tissue
Figure 3 IHC staining of human skeletal muscle
Low expression. RNA expression: 34.0 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_B_3_6_val_selected.jpg
Normal Tissue
Figure 4 Colon
Mucosal lymphoid cells
Staining: Low
Intensity: Moderate
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55/231_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48284/124814_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000090382-LYZ
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Specifications
- Immunogen
- Chicken lysozyme (renal amyloidosis)
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG
- Specificity
- Chicken LYZ
- Species Reactivity
- Chicken
- Clone
- HuLys11
- Applications
- WB, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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(Creative Biolabs Cat# PABW-023, RRID: AB_3111681)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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|---|---|---|---|
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| PSBZ-015 | Mouse Anti-LYZ Recombinant Antibody (clone HyHEL-5); scFv Fragment | WB, FuncS | Mouse scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-073 | Recombinant Human Anti-Lysozyme Antibody (HuLys) | WB, ELISA, FuncS | IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-139 | Mouse Anti-LYZ Recombinant Antibody (PABX-139) | WB, ELISA, FuncS | Mouse IgG |
| PABX-073-S (P) | Recombinant Human Anti-Lysozyme Antibody scFv Fragment (HuLys) | WB, ELISA, FuncS | scFv |
| PABX-074-S (P) | Recombinant Mouse Anti-Lysozyme Antibody scFv Fragment (PABX-074-S (P)) | WB, ELISA, FuncS | scFv |
| PABX-074-F (E) | Recombinant Mouse Anti-Lysozyme Antibody Fab Fragment (PABX-074-F (E)) | WB, ELISA, FuncS | Fab |
| PABX-139-F (E) | Mouse Anti-LYZ Recombinant Antibody; Fab Fragment (PABX-139-F (E)) | WB, ELISA, FuncS | Mouse Fab |
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